Desktop Software for Patch-Clamp Raw Binary Data Conversion and Preprocessing
نویسندگان
چکیده
منابع مشابه
Automated planar patch-clamp.
Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as controlling many metabolic functions. Furthermore, i...
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Patch-clamp recording has enabled single-cell electrical, morphological and genetic studies at unparalleled resolution. Yet it remains a laborious and low-throughput technique, making it largely impractical for large-scale measurements such as cell type and connectivity characterization of neurons in the brain. Specifically, the technique is critically limited by the ubiquitous practice of manu...
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To test the hypothesis that Ca channel plays a role in renal epithelial Ca transport, we exposed and patched apical membranes of freshly microdissected rabbit connecting tubules (CNTs). Single channel Ca currents were recorded with Ba as the charge carrier. In the cell-attached mode, 8-Br-cAMP increased the open-state probability (P.) to 0.6%. In excised, inside-out patches, P. was low spontane...
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The patch-clamp recording technique, which measures ionic currents under voltage clamp, was designed to study small patches of membranes in which near perfect control of the transmembrane voltage can be achieved readily. The recent application of patch-clamp methodology to the analysis of whole-cell current actually defies many of the original design requirements. Nevertheless, whole-cell recor...
متن کامل"MS-Patch-Clamp"or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics
In this projecting work we propose a mass spectrometric patch-clamp equipment with the capillary performing both a local potential registration at the cell membrane and the analyte suction simultaneously. This paper provides a current literature analysis comparing the possibilities of the novel approach proposed with the known methods, such as scanning patch-clamp, scanning ion conductance micr...
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ژورنال
عنوان ژورنال: Journal of Electrical and Computer Engineering
سال: 2011
ISSN: 2090-0147,2090-0155
DOI: 10.1155/2011/251215